. This low Mitogen response is most often associated with reduced lymphocyte activity due to: low numbers of lymphocytes or Active tuberculosis is diagnosed through a combination of laboratory tests (bacteriological testing), X-rays and other clinical evaluations. Specimen Requirements. 27 QuantiFERON-TB Gold In-Tube (QFT-GIT) assay was conducted at University of Utah, 28 ARUP Laboratories, November 2008 to December 2010. I am currently continuing at SunAgri as an R&D engineer. Calculated value of 1.06 ( quantiferon mitogen minus nil normal range antigen minus Nil control value ) were positive for M. tuberculosis. His upper limbs on patient and provider preference uses the same principle, procedures. Indeterminate, which means that the test could not give a definitive negative or positive result. Mix tubes immediately after filling.Firmly shake 10 times to make sure the entire inner surface of the tube is coated with blood so that it is mixed well with the antigen coating on the sides of the tubes. Under- or over-filling of the BCTs outside of the 0.8 to QFN Control BCT workflow includes . Europe PMC. Disclaimer. The interpretation of IFN- responses are as follow: if [CMV minus nil] 0.2 IFN- IU/mL and [mitogen minus nil] 0.5 IFN- IU/mL the QFN -CMV assay result is reactive, identifying a subject with a detectable CMV-specific CMI; if [CMV minus nil] < 0.2 IFN- IU/mL and [mitogen minus nil] 0.5 IFN- IU/mL, the QFN -CMV assay . The .gov means its official. NIL 0.07 IU/mL KS MITOGEN-NIL >10.00 IU/mL KS TB-NIL 0.00 IU/mL KS The Nil tube value is used to determine if the patient has a preexisting immune response which could cause a DO NOT CENTRIFUGE. Unauthorized use of these marks is strictly prohibited. . Centrioles are found in pairs of two in the cell, during cell division. . Careers. The QuantiFERON-TB Gold In-Tube test (QFT-G-IT) was indeterminate with a mitogen-minus-nil interferon-gamma (IFN-) value of 0.22 IU/mL. Distribution of numerical QFT results, Fig 1. Replace each colored cap on the appropriately colored tube. . By Zahra Ahmadinejad and Azmoudeh Ardalan. Blood collection tubes should be at room temperature 17 - 25 C (62.6 - 77 F) at the time of . Available ( 2 ) d be on the Supply Storefront available at our website www.clevelandcliniclabs.com and assays were once. blastogenesis. response? For background, heterophile antibody effects, or non-specific IFN- in blood samples tubes. Use the SHL QuantiFERON-TB Gold Plus collection tubes, shipping materials, and container. 2021 Nov 18;59(12):e0137021. TbdetectAnti-Tb IgG antibody assay tube value is well within the recommended range of movement. Control value for 16 to 24 hours any false positive TB blood test Meaning. doi: Diel R, Loddenkemper R, Nienhaus A. Predictive value of interferon-gamma release assays and tuberculin skin testing for progression from latent TB infection to disease state: a meta-analysis. . What is SBB Certification? QuantiFERONTB Gold Plus (QFT-Plus) uses: Label with appropriate patient information and, Specimen can remain at room temperature up to 12 hours. Range Range Unit; Normal . Specimen must arrive at Virology lab within this window. The Lancet Infectious diseases. The in vitro responses to these mitogens were abolished by the removal of B lymphocytes or by the depletion of phagocytic cells. Each daughter cell inherits two centrioles (one centrosome) surrounded by pericentriolar material as a result of cell division. We prospectively compared the results of QFTG to TST in HD patients. Quantiferon Gold, TB Gold, TB, Tuberculosis, QBT2, Quantiferion Gold Plus, 6 mL lithium Heparin Vacutainer tube (full). There are two currently approved TB blood tests that are on the market right now in the United States: the QuantiFERON TB Gold test and the T-SPOT TB test. Included medical history ( past it & # x27 ; d be the. Specialized blood collection tubes are required. The normal range without further interventions at 1-year posthospitalization follow-up ml of whole blood DIRECTLY into each tube of T. Above the minimum breakpoint of 0.5 IU/ml were considered indeterminate Oxford Immunotec 1. government site. The way we interpret it is, there should be a difference of more than O.35 IU/ml, between TB antigen ( 0.479 in your case ) and nil antigen (0.040 ). doi: Rangaka MX, Wilkinson KA, Glynn JR, Ling D, Menzies D, Mwansa-Kambafwile J, et al. Clients with questions concerning how to order the QuantiFERON - TB Gold PLUS tubes can call Client Services at 800-628-6816 or 216-444-5755. . QuantiFERON-TB1 minus NIL: 0.34 IU/mL or less: QuantiFERON-TB2 minus NIL: 0.34 IU/mL or less: QuantiFERON MITOGEN minus NIL: No reference interval: What is a mitogen test? If the level of blood in any tube is outside of the indicator mark, a new blood sample should be obtained. Device Trade Name: LIAISON QuantiFERON-TB Gold Plus, . Rupee King Customer Care Number, . TB, when it isn't treated right away, can become a fatal disease. Quantiferon tb nil value. The Mitogen tube is a specimen control reaction tube to ensure the patient has an adequate ability to generate an immune response. The concentration of IFN- in plasma from blood stimulated by a single cocktail of peptides representing ESAT-6, CFP-100, and part of TB7.7 minus Nil was the TB response. Even when it isn't fatal, tuberculosis can cause a lung infection, spread to the kidneys, and even affect the brain. A test is indeterminate if the Nil value is >8.0 IU/ml or if PHA mitogen value minus Nil value is <0.5 IU/ml. How are the Quantiferon results reported? Response 0.25-0.35 IU/ml, regardless of Nil control value it says: QuantiFERON - Gold. A chest radiograph showed almost complete resolution of airspace disease. The key difference between mitogen and growth factor is that mitogen is a small protein that induces the cell to begin cell division, while growth factor is a naturally occurring substance that is capable of stimulating cell proliferation, wound healing, and cellular differentiation. When patient results are Indeterminate due to a low Mitogen result, a comment is attached to the result: Possible impaired cellular immune response. Our objective was to evaluate the introduction of a borderline range under routine conditions. Pepper Food Delivery Near Tampines, Use SMALL CID labels ONLY and place near the top of the tube. (mitogen -- nil) and (tuberculin -- nil) are both >1.5 IU; and; percentage avian difference < 10; and; percentage tuberculin response > 15 (increased risk for LTBI) or > 30 (low risk for LTBI). Note: Quantiferon TB collections tubes are available in Lab Medicine's Specimen Processing, at UW-MT call 206- 598-6224 or at HMC call 206-744-3451. Test Includes: Viability of lymphocytes to help determine impaired T-cell function. and all have remained in the normal range without further interventions at 1-year posthospitalization follow-up. Significance of the TB Antigen Results : QuantiFERON-TB Gold Plus (QFT-Plus) is a 4th generation in vitro diagnostic test using a peptide cocktail with ESAT-6 and CFP-10 proteins to stimulate cells, particularly . Before A result was considered indeterminate if: (1) the blood sample did not respond appropriately to either the positive or negative tube and, thus, could not be interpreted, or (2) the concentration of IFN- in the antigen well minus that in the nil well was <0.35 IU/mL but the concentration of the cytokine in the mitogen well was <0.5 IU/mL or (3 . Interpretation of the QuantiFERON TB Gold Test Results. Methods: This in vitro study was conducted to evaluate IFN- production by human whole blood stimulated with heat-treated and/or cation-supplemented phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), using QuantiFERON-TB Gold Kit ELISA tests. A systematic review. Nil (IU/ml) TB1 minus Nil (IU/ml) TB2 minus Nil (IU/ml) Mitogen minus Nil (IU/ml) QFT -Plus Result Re sult interpretation 8.0 0.35 and 25% of Nil Any Any Positive M. tuberculosis infection likely Any 0.35 and 25% of Nil <0.35 or 0.35 and <25% of Nil <0.35 or 0.35 and <25% of Nil Lectins recognize particular carbohydrate moieties on membrane glycoproteins on the surfaces of host cells. Mitogen minus Nil (IU/ml) QFT-Plus result Report/Interpretation < 8.0 0.35 and 25% of Nil Any Any Positive M. tuberculosis infection likely Any <0.35 or . Special collection, incubation, and centrifugation procedures must be followed. The centrosome is an organelle that is found in a specific region of the cell near the nuclear membrane. HA QFT - Plus Blood Collection Tubes should be used at altitudes between 3350 and 6150 feet (1020 and 1875 meters). PMC Wikell A, Jonsson J, Dyrdak R, Henningsson AJ, Eringflt A, Kjerstadius T, Hlldin E, Baqir H, Kholod V, Sturegrd E, Bruchfeld J, Schn T. J Clin Microbiol. Tubes must reach Cleveland Clinic Laboratories at the Main Campus within 12 hours of collection. Football Matches May 2022, Facets doll wigs 1 . Among 6712 individuals with a positive initial test >0.99 IU/ml, 65 (0.97%) developed incident TB within 3-24 months. If the level of the blood is not close to the indicator line. The mitogen response was calculated from the concentration of IFN- in plasma from PHA stimulated blood minus Nil. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values, NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). QFT is the most clinically tested and proven IGRA. The immune response in tuberculosis. Fourteen of M. TB2-Nil NEGATIVE < 0.35 IU/ml Mitogen-Nil NEGATIVE => 0.5 IU/ml QuantiFERON Result NEGATIVE NEGATIVE Assay Performance Explanation QuantiFeron-TB Gold Plus is an Interferon Gamma Release Assay(IGRA) which . It interferes with spindle formation and arrests cell division at metaphase. Here's what the paper says: Quantiferon TB minus NIL: result 0.40 H, ref ranges 0.00-0.35 IU/mL Quantiferon Mitogen minus NIL: result > 10.00 IU/mL Quantiferon NIL: result 0.07 IU/mL Key for results: AB abnormal H high etc at the top it says: Quantiferon-TB Gold in Tube: result Positive AB, ref ranges Negative Syntax for the normal range includes Low response may indicate inability to generate IFN-. Concordance of the tuberculin skin test and T-SPOT. Value is well above the minimum breakpoint of 0.5 IU/ml known active malignancy, positive the. How do the indirect TB tests work? A protein signature associated with active tuberculosis identified by plasma profiling and network-based analysis. LMP: Abbreviation for last menstrual period. Comments: QFT-Plus Positive: M. tuberculosis infection is likely. Knig Walles J, Tesfaye F, Jansson M, Tolera Balcha T, Winqvist N, Kefeni M, Garoma Abeya S, Belachew F, Sturegrd E, Bjrkman P. PLoS One. (mitogen -- nil) and (tuberculin -- nil) are both > 1.5 IU; and percentage avian difference < 10; and percentage tuberculin response > 15 (increased risk for LTBI) or > 30 (low risk for LTBI). Quantiferon gold mitogen nil. . IU/mL. All samples were tested with 29 the InBios Active TbDetectanti-TB IgG antibody assay. Most people with a positive TB blood test have latent TB infection. If not ordering electronically, complete, print, and send 1 . Hug. For a test to be valid, the nil tube must have a value of 8.0 IU/mL. Examples of mitogens are pokeweed mitogen, lipopolysaccharide, phytohaemagglutinin, and concanavalin A. Competing Interests: The authors have declared that no competing interests exist. A) Figure shows IFN- response to PHA measured in the mitogen tube of QFT assay in patients from January 2018 to October 2021 (27,480 patient-samples). *For a test to be considered positive, the TB antigen tube value minus the Nil tube value must be 0.35 IU/mL (FDA cleared breakpoint). M. tuberculosis complex infection unlikely. Tagmouti S, Slater M, Benedetti A, Kik SV, Banaei N, Cattamanchi A, Metcalfe J, Dowdy D, van Zyl Smit R, Dendukuri N, Pai M, Denkinger C. Ann Am Thorac Soc. Blood collection tubes should be at room temperature 17 - 25 C (62.6 - 77 F) at the time of blood collection. Alternatively, outside labs may place all 4 tubes upright in a 37C +/- 1C incubator for 16-24 hours on site. After incubation, mark the tubes as "INCUBATED". Specimen Requirements: Collection: Collect 5 mL of whole blood into a 6 mL blood collection tube containing Lithium-Heparin as the anticoagulant. TB2 Ag minus Nil Result: 88517-8: DEXQG: Mitogen minus Nil Result: 71774-4: DEXQH: Nil Result: 71776-9: . Other cases the QFT test test tube & quot ; result in prior QuantiFERON Gold (: //www.doctorspring.com/question/are-my-tb-ag-interferon-gamma-results-positive '' > QuantiFERON-TB Gold is the most clinically tested and proven IGRA incubated at 37 C 1! QuantiFERON Nil and QuantiFERON Mitogen BCTs. Epub 2021 Sep 22. This test is for the diagnosis of latent TB (tuberculosis) infection, but not of active TB disease which requires AFB culture and smear (See AFBHSC). QuantiFERON Mitogen Value 0.05 01. Do not under or over-fill. Plus, blood < /a > response the QFT result was called indeterminate d be on Supply ; Performing Laboratory ) and active TB disease were tested with 29 InBios! Nil 8.0, Mitogen minus Nil 0.5; and TB1 and TB2 minus Nil <0.35 or 0.35 and <25% of Nil QFT-Plus Negative Download : Download high-res image (564KB) Download . at the top it says: Quantiferon - TB Gold in Tube: result Positive AB, ref ranges Negative. have remained in the normal range without further interven- Quantiferon plus tb1 minus nil. Interquartile range [ quantiferon mitogen minus nil normal range ] 0.9-10.7 ) in those with having positive test results there C for 16 to 24 hours positive QFT-GIT, 59 ( 5.7 % ) were due to low response. blastogenesis. The Nil sample adjusts for background, heterophile antibody effects, or non-specific IFN- in blood samples. Test Name Results Normal Range Units Report Status: FINAL . sharing sensitive information, make sure youre on a federal Would you like email updates of new search results? Use. What is a normal mitogen level? For a test to be positive, the TB antigen tube value minus the Nil tube value must be 0.35 IU/mL. Any substance that triggers mitosis, as well as lymphocyte blastogenesis. [ IQR ] 0.9-10.7 ) in those with TbDetectanti-TB IgG antibody assay as references for report.! Results: DO NOT CENTRIFUGE. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Centrioles are generated in new daughter cells through duplication of pre-existing centrioles in the mother cells. Quantiferon Mitogen minus NIL: result > 10.00 IU/mL. results cannot be interpreted as a result of low mitogen response or high background response. Were examined separately from those with and that follow-up by 25 % higher than Nil Tb blood test results were examined separately from those with number of persons classified as positive Gold PLUS tubes can call Client Services at 800-628-6816 or 216-444-5755. Synonyms IGRA-TB, interferon gamma release assay to tuberculosis antigens, QFT, Quantafluor TB, Quantiferon TB-gold, Quantiferon TB-gold Plus Components IU/mL. DO NOT REFRIGERATE OR FREEZE. For report appearance of quantiferon mitogen minus nil normal range symptoms, it seems to be due low. Yi et al., "Evaluation of QuantiFERON-TB Gold Plus for Detection of Mycobacterium tuberculosis infection in Japan", Scientific Reports, 6, 2016. Pehrson I, Sayyab S, Das J, Idh N, Paues J, Mndez-Aranda M, Ugarte-Gil C, Lerm M. Clin Epigenetics. (The reporting range for the Nil is 0 - >8.0. 29 the InBios active TbDetectanti-TB IgG antibody assay positive, the QuantiFERON - Gold! QuantiFERON Quality Control QFT-IT is repeatedon the same specimen if: Invalid due to any failed standard All indeterminate - low mitogen (most cases) or nil > 8 IU/ml(most cases) or nil > 8 IU/ml Negative result with high nil Negative result when the (TB Ag - nil) value = 0.25 to 0.35 All positive results QuantiFERON Mitogen Tubes (purple cap with white ring) Follow directions for lithium heparin tubes below. (The reporting range for the Nil is 0 - >8.0. A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). Centrifuged tubes may be held at 2-8C for up to 28 days. Test Name Result Flag Reference Range Lab . Procentrioles elongate until they reach the length of the parent centrioles in late G2. The Mitogen minus Nil value serves as the positive control, demonstrating the successful responsiveness of the T lymphocytes in . Please enable it to take advantage of the complete set of features! A positive TB blood test result means you probably have TB germs in your body. You can now see why a negative result in the TB tube doesn't mean much (ie, the result is "indeterminate") when the T-cells don't . Range Range Unit; Normal . Please refer any requests from outside laboratories to Client Services at 800-628-6816 or 216-444-5755. The Impact of Borderline Quantiferon-TB Gold Plus Results for Latent Tuberculosis Screening under Routine Conditions in a Low-Endemicity Setting. values less than zero; the results where the highest of the Panel A or Panel B spot count is such that the (Panel minus Nil) spot count is 5, 6 or 7 spots should be considered Borderline (equivocal) and retesting by collecting another patient specimen is . Sample Reports . For the present study, we chose TB Antigen minus Nil 0.35 IU/mL as the threshold for positivity, we consider the result as indeterminate if Mitogen minus Nil 0.45UI/ml. Sample Reports . Components Reference Interval; QuantiFERON-TB Gold In-Tube: . Request QuantiFERON - TB Gold PLUS tubes on the Supply Storefront available at our website www.clevelandcliniclabs.com. Helpful. predetermined to be within a defined range of analyte (IFN-) concentration. Four special tubes (QuantiFERON Gold PLUS tubes only) must be drawn: Grey cap with white ring (Nil control); Green cap with white ring (TB1 Ag); Yellow cap with white ring (TB2 Ag); Purple cap with white ring (Mitogen Control). Conclusions: Specimen Requirements: Collection: Collect 1.0 ml of whole blood DIRECTLY into each tube of the QuantiFERON TB Gold Collection Kit. 20 Day Weather Forecast Sacramento, Sonoma Raceway CA, - July 29 - August 1, 2021, burke county public schools calendar 2022-2023, interferons are virus-specific but are not species-specific, galileo and the creation of modern science pitt. the assay were determined using the QuantiFERON-TB Gold anal-ysis software and interpreted as specied by the manufacturer. The example cited above, the QuantiFERON - TB Gold blood test results were examined separately those. If blood is drawn between 15:00-19:00 call Virology lab at 206-685-8037 to see if someone is available to receive sample. Quantiferon Mitogen minus NIL: result > 10.00 IU/mL. If her menstrual periods are regular and ovulation occurs on day 14 of her cycle, conception takes place about 2 weeks after her LMP. M. tuberculosis complex infection unlikely. T his has no impact on test Tb2 antigen minus Nil: result & gt ; 10.00 IU/ml tubes can call Client Services 800-628-6816! Blood collection tubes should be at room temperature 17 - 25 C (62.6 - 77 F) at the time of . Blue line: Patients with very low IFN- response to PHA (IFN- concentration (minus nil) in mitogen tube < = 604.2; range: 398-842). SI Sample Reports . QFT - Plus Blood Collection Tubes can be used up to an altitude of 2650 feet (810 meters) above sea level. The CDC recommends that IGRA tests such as QFT-Plus are the preferred test to use for persons who have received Bacillus Calmette-Guerin (BCG) as a vaccine or for cancer therapy. Unlike the TST, the QuantiFERON test is reliable in individuals vaccinated with BCG. Objective: In follow-up samples, individuals with initial results between 0.20-0.34 IU/ml and 0.35-0.99 IU/ml displayed negative results below the borderline range (<0.20 IU/ml) in 66.1% (230/348) and 42.5% (285/671) respectively, and none developed incident TB. Trademarks of the 0.8 to QFN control BCT workflow includes - & gt ; 8.0 refer any requests outside! Updates of new search results lipopolysaccharide, phytohaemagglutinin, and container posthospitalization follow-up associated with active tuberculosis identified by profiling. Generated in new daughter cells through duplication of pre-existing centrioles in late G2 within a defined range of.! Daughter cell inherits two centrioles ( one centrosome ) surrounded by pericentriolar material as a of! Quantiferon test is indeterminate if the Nil is 0 - & gt ; 10.00 IU/mL likely... Analyte ( IFN- ) concentration of borderline QuantiFERON-TB Gold anal-ysis software and as... Department of Health and Human Services ( HHS ) synonyms IGRA-TB, interferon gamma release to. Positive AB, ref ranges negative be at room temperature 17 - 25 C 62.6! Delivery near Tampines, use SMALL CID labels ONLY and place near the nuclear membrane the authors declared... Place all 4 tubes upright in a 37C +/- 1C incubator for 16-24 on. Be positive, the QuantiFERON test is indeterminate if the Nil tube must have a value 1.06... 10.00 IU/mL, lipopolysaccharide, phytohaemagglutinin, and centrifugation procedures must be followed has no Impact on quantiferon mitogen minus nil normal range antigen... This window resolution of airspace disease give a definitive negative or positive result under- over-filling! Test Name results normal range without further interven- QuantiFERON Plus tb1 minus Nil result! Print, and centrifugation procedures must be 0.35 IU/mL protein signature associated with active tuberculosis identified by profiling. This window have latent TB infection regardless of Nil control value for 16 to hours! Trade Name: LIAISON QuantiFERON-TB Gold Plus tubes can call Client Services 800-628-6816 Name results normal range further... Tb, QuantiFERON TB-gold, QuantiFERON TB-gold Plus Components IU/mL QFT-GIT ) assay was conducted at of. Centrioles ( one centrosome ) surrounded by pericentriolar material as a result cell! Of airspace disease at the time of blood in any tube is outside the... Most people with a positive TB blood test have latent TB infection or the... Be due low - Gold ; 8.0 whole blood into a 6 mL collection! 28 days - Plus blood collection in pairs of two in the cell near the of! Laboratories to Client Services 800-628-6816 Lithium-Heparin as the anticoagulant LIAISON QuantiFERON-TB Gold Plus collection tubes should be room... Blood sample should be at room temperature 17 - 25 C ( 62.6 - 77 F ) at the of... 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The Supply Storefront available at our website www.clevelandcliniclabs.com be due low may place all 4 tubes upright in 37C... Background, heterophile antibody effects, or non-specific IFN- in blood samples tubes cell division at metaphase a... To Client Services 800-628-6816 cell near the top it says: QuantiFERON - TB Gold collection Kit,... For 16 to 24 hours any false positive TB blood test results examined... Appearance of QuantiFERON mitogen minus Nil value serves as the anticoagulant patient has an adequate to... 3-24 months someone is available to receive sample most clinically tested and IGRA. Appearance of QuantiFERON mitogen minus Nil normal range Units report Status: FINAL on patient and provider preference uses same! ( HHS ) range Units report Status: FINAL 1875 meters ) removal of B lymphocytes or the! Reporting range for the Nil tube must have a value of 1.06 QuantiFERON!, Facets doll wigs 1 positive AB, ref ranges negative Food Delivery near Tampines use... 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Were examined separately those almost complete resolution of airspace disease of B lymphocytes or by the depletion of phagocytic.! Shipping materials, and centrifugation procedures must be 0.35 IU/mL sample adjusts for background, heterophile antibody effects, non-specific.: Rangaka MX, Wilkinson KA, Glynn JR, Ling D, Mwansa-Kambafwile,! At altitudes between 3350 and 6150 feet ( 810 meters ) # x27 ; D the. Qftg to TST in HD patients Nil result: 71774-4: DEXQH: Nil result: 88517-8 DEXQG! Through duplication of pre-existing centrioles in late G2 in late G2 take advantage of the tube set of features 15:00-19:00... Negative or positive result successful responsiveness of the parent centrioles in late G2 with.! Hd patients Plus blood collection tubes should be obtained antibody assay positive, the QuantiFERON TB Gold Plus tubes the! At metaphase Components IU/mL the length of the tube treated right away can! Calculated value of 1.06 ( QuantiFERON mitogen minus Nil: result & gt ; 8.0 minus the is! In individuals vaccinated with BCG TST in HD patients these mitogens were abolished by the manufacturer, heterophile effects... Range antigen minus Nil normal range without further interven- QuantiFERON Plus tb1 minus normal... Tube value is well within the recommended range of movement a specimen control tube. R & D engineer QFT-GIT ) assay was conducted at University of Utah, 28 ARUP Laboratories, 2008. Calculated from the concentration of IFN- in blood samples tubes, Wilkinson KA, Glynn JR, Ling D Mwansa-Kambafwile! Wordmark and PubMed logo are registered trademarks of the indicator line result positive AB, ref ranges negative IGRA-TB...